Based Hygiene Monitoring
Protein Based Hygiene
Dipslides and Incubators
ATP Monitoring Systems
& Enrichment Products
International Plc is a
leading manufacturer and supplier of specialist microbiology and life
science products and services. The company has leading brands such as
Biotrace, Cogent, Ruskinn, International BioProducts and Lucigen.
acknowledged as the global leaders in Rapid Hygiene Monitoring in the
food processing, food retail and catering markets. Many of the world's leading
food manufacturers and catering companies now rely on the Biotrace products as
an essential part of their quality procedures. Rapid tests and consistent
results ensure that Biotrace hygiene monitoring systems are also the choice for
environmental and industrial processes such as paper/pulp biocide treatments and
cooling water tower assessments. Customers include world leading water treatment
chemical multi-nationals as well as companies within the cosmetics, toiletries
and pharmaceutical sectors. Another product line that utilises ATP technology is
that of the Defence/Bio-Detection Products. Biotrace
has a dedicated team designing and providing state of the art
detection systems to continuously monitor in real-time, the levels of airborne
the way in ATP-bioluminescence for end product screening in the UHT dairy and
fruit juice markets.
Technology, which became
part of the Biotrace Group in November 2001, is the market leader in anaerobic and
modified oxygen incubation systems and workstations.
Established in 1993, Ruskinn has focused entirely on innovative new
products in this specialist area. Today, the Ruskinn brand is the product choice
of microbiologists and medical researchers in over 30 countries.
International, serves a wide
customer base in healthcare, industrial and defence markets.
CLEANLINESS TESTING AND HYGIENE MONITORING:
cleanliness testing systems measure the levels of product residue and microbial
contamination once cleaning has taken place. High levels of product residue
will indicate inadequate cleaning and contamination risk. Biotrace
BASED HYGIENE MONITORING:
(Adenosine Triphosphate) is a universal energy molecule common to all living
cells and organisms. ATP is therefore present in food residues as well as
bacteria. It was found that the firefly produces its’ characteristic glow by
utilising ATP in a reaction with the luciferase enzyme and luciferin substrate
to release energy as light. Utilising the firefly substrate and enzyme, Biotrace
designed their reagents to produce light in the presence of ATP. The light
produced by the reagents can then be measured in a light sensitive luminometer (Unilite
or the Unilite NG).
ATP bioluminescence has become the industry’s gold standard for rapid hygiene
sets the standard for rapid surface hygiene testing as this simple
‘pen-like’ device contains all the necessary reagents in a single shot
format. Testing could not be easier. Just swab, activate and measure.
Clean-Trace can determine within 30 seconds whether it is safe for production to
Aqua-Trace (Water Testing)
is designed to rapidly
assess the cleanliness of CIP rinse waters by detecting for the presence of
product residues and microbial contamination. Aqua-Trace is ideal for closed
manufacturing equipment where CIP water is used as an indicator of cleanliness.
The user-friendly single shot device can be used in production areas with
minimal training required. Aqua-Trace is available as a total test to determine
total levels of ATP from both product residue and microbial cells.
Alternatively, an additional ATP free test will provide only somatic ATP levels.
The value obtained from the ATP free test can be subtracted from the total ATP
value to provide microbial ATP levels.
Bev-Trace (End Product
Bev-Trace is a rapid test kit for the determination of microbial
contamination in filterable beverages. The method requires multiple steps and
results are obtained within 24 hours, therefore reducing goods holding times and
saving costly warehousing space. The Bev-Trace kit is also ATP based and
is read using the Biotraceâ
has two luminometers available:
A basic luminometer, used in conjunction with the Biotrace swabs, to determine
surface and CIP water contamination levels within seconds. Hygiene testing could
not be simpler than 3 steps. Insert the swab, close the chamber and read.
Once the instrument has read the sample, it generates results in relative light
units (RLU) on the LCD display, and a results report will be printed via the
external thermal printer.
The Uni-Lite NG (Next Generation) Luminometer from Biotrace boasts many
valuable features. The NG has the honour of being the lightest PMT
(photomultiplier tube) based luminometer on the market, giving you sensitivity
with portability. The Uni-Lite NG offers advanced data
trending and re-test facilities with it's windows based software called
Bio-Track+. Used with Clean-Trace and Aqua-Trace swabs to determine
the level of contamination in a sample, results are available in 30
seconds. The NG docking station is ideal for desk top stability and gives
instant connection to your PC and charger, without the hassle of plugging or
Bio-Track+ Data Trending Analysis Software
+ Software is the most powerful hygiene data trending software available.
Bio-Track+ allows the creation of sample plans (a plan of which points you want
tested and their pass/fail levels), and the upload of these plans to your
Uni-Lite NG Luminometer. Once testing has been completed, download data
from your Uni-Lite NG to your PC for further data reduction.
Bio-Track+ software can be as simple or sophisticated as you want or need it to
be. Whether you require sample measurements only or the system to be
integrated as part of your HACCP monitoring programme, providing management
reports, the Biotrace system is the system for you.
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BASED HYGIENE TESTING SYSTEMS:
It is a quick, simple and cost effective device that provides instant visible
results based on the detection of protein residues left from improper cleaning.
Simply open the swab packet, expose the swabs angled tip, swab the surface,
place swab in the swab packet and burst the yellow reagent onto the angled tip.
Observe the instant colour reaction. A yellow colour indicates that
cleaning was effective but a blue/green colour is indicative of inadequate
cleaning. The Check It Swab can only detect protein residues and is
ideally suited for use in the meat, fish and dairy industries.
Pro-Tect is a superior swab to that of the Check It device. It is easier to use and
detects a wider variety of food residues. Protect can detect
both protein and reducing sugars. The self-contained one step device utilises a
simple colour change to indicate surface cleanliness. The swab can determine up
to four levels of contamination. Green indicates clean, grey indicates caution
(advise re-rinsing), purple indicates a dirty surface and deep purple indicates
a heavily contaminated dirty surface (re-cleaning). This product does not
necessarily require an instrument but for quantitative results it can be used in
conjunction with the Pro-Lite
is designed to be used with Pro-Tect swab. The instrument measures the
colour change reaction and provides numerical results in relative colour units (RCU’s).
The printer provides a hard copy of data for record keeping and associated HACCP
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DIPSLIDES AND INCUBATORS:
The Dipslide is a safe,
reliable and fast indicator of bacterial contamination in liquids and on
surfaces. The slide is supplied in a sterile container and is pre-coated with
selected media on either side. The sterile slides can be used on site by simply
removing the slide from the sterile container, immerse it in the solution for
liquid testing or press the slide onto contact areas for surface testing. Once
the sample has been taken, the slide is placed back in the sterile container and
incubated. After an incubation period, the slide can be examined for growth and compared to the
model chart provided. The Dipslides have an extended shelf life of nine months
and are available as single or dual tests.
Specific Dipslides are available with a
neutralising agent added to help cope with the effects of sanitizers used on
surfaces after cleaning. It is not necessary to use a Dipslide with a
neutraliser, but it can be useful as a precautionary measure. Certain
Dipslides are also available with Redspot, which is the name given to Dipslides
that contain TTC. TTC is a colourless chemical that is turned red by most
bacteria, making the colonies easier to count.
following slide formats are available:
||Total Viable Count (TVC) with
||Nutrient Agar + TTC (X2)
||TVC with Redspot and
||Plate Count Agar (PCA) + TTC
||TVC with Redspot / Coliforms
||PCA + TTC/Macconkey 3
||TVC with Redspot / Coliforms
||PCA / Macconkey 3 +
||TVC with Redspot / Yeasts and
||Nutrient Agar + TTC / Malt
||TVC / Yeasts and Moulds
||Tryptone Soy Agar (TSA) /
Rose Bengal Agar
||Yeasts and Moulds
||Rose Bengal Agar (X2)
||TVC / Yeasts and Moulds with
||PCA / OGYE + Neutraliser
||TVC / Enterobacteria
||PCA / Violet Red Bile Glucose
Agar (VRBG) + Neutraliser
||TVC / Enterobacteria
||PCA / VRBG
||Baird Parker Agar / VRBG
Incubators are easy to use, durable, reliable and compliment the Biotrace
range of Dipslides. Depending on your requirements you can choose between the
Almedica incubator or the portable Veba MediTemp incubator.
static incubator, the Almedica is a small multi-purpose incubator ideal for on-site
With a capacity to hold up to 24 Dipslides, it can be free standing or
wall mountable, making it ideal for laboratories of all sizes.
with a thermometer, the temperature
range is ambient +50C from 25
- 450C (+ / - 10C).
The portable Veba MediTemp, provides
the ideal solution for customers when incubating samples ‘off-site’ is a
priority. Veba MediTemp is a perfect incubator for multi-site companies,
universities or research centres where field work is carried out or companies
with no dedicated laboratory services on site. The
Veba MediTemp has 10 litre capacity and can be used from the mains or plugged
into a car cigarette lighter socket.
This enables the incubation process to start as soon as a sample is
taken, with no need for laboratory facilities. Temperature is 350C
standard, but it can be set at 30 - 370C at time of order.
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ATP BIOLUMINESCENCE SYSTEMS:
The Microbial Luminescence
system (MLS) for UHT End Product Sterility Testing
has proven to be an effective, highly accurate method for testing UHT products
in comparison to traditional ph readings and culture methods. Cogent
Technologies a division of Biotraceâ
ATP-bioluminescence to detect the presence of microbial ATP in UHT dairy, as
well as non-dairy products such as rice, soy ‘milks’ and fruit juices. Used
in conjunction with the Microbial Luminescence System (MLS), the UHT product
screening reagents are specifically designed to exclude ATP from non-microbial
sources, thereby measuring only microbial ATP in Relative Light Units (RLU). The
measurement of microbial ATP indicates the presence of microbial contamination
in the product. The three steps involved are incubation, sample transfer to
microwells and finally placement of the microplate into the MLS system for
reagent addition and sample analysis. The advantages
of ATP testing in UHT products are as follows; high correlation to traditional
methods with results available 48 hours faster, easy to perform tests and
earlier detection of lactobacillus, assured consistency between technicians and
plants, manufacturing costs are lower by reducing goods holding time and
is a unique in-line system used to automatically assess microbial contamination
in liquid or air samples by utilising ATP bioluminescence technology. The system
continuously samples the liquid or air and determines microbial ATP by
calculating the difference between total (non-microbial + microbial) and free
(non-microbial) ATP. The system utilises two detection channels to determine
total and free ATP levels. The system can be used to continuously monitor CIP
water in the food industry or to monitor biocide activity in the paper and pulp
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ATP MONITORING SYSTEMS:
BBDS is a modular non-specific airborne biological detection system, designed
for use as a state-of-the-art, early warning system.
Air Sampler utilises wet walled cyclone technology for continuous detection of
airborne biological contamination.
The Continuous Flow ATP
CFAD is a unique system, using patented technology to detect microbial
contamination in a liquid sample using ATP bioluminescence technology.
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was the first to introduce the world’s smallest anaerobic workstation,
together with the rapid transfer interlock and single plate entry systems. They
were also the first to provide the convenient “bare hand” Ezee sleeve™
system and intelligent humidity control. It is therefore easy to see why Ruskinn
systems are the choice of many microbiologists and research scientists around
the world. Instruments available include:
The world’s favourite compact anaerobic workstation. Perfect for the smaller
microbiology laboratory or as a personal workstation, the popular BugBox shares
the innovative features of the larger Ruskinn workstations yet takes up a
smaller area on the workbench. The BugBox is also available for microaerophilic
testing. The BugBox M has all the standard features but can interlay with the
Ruskinn patented gas mixing system.
With a spacious 400 plate working capacity, the Concept 400 is the choice of
many hospital and general microbiology laboratories. Providing a perfect
combination of capacity, speed and innovation, the concept 400 delivers simple
sophistication and new levels of comfort and convenience. The Concept 400 can
also be used for microaerophilic testing. The Concept 400-M incorporates all the
familiar and proven features of the Concept 400, but it is tailored to interface
with the Ruskinn patented gas mixing system.
The Concept Plus is designed to meet the needs of the larger laboratories, where
sample throughput and incubation capacity is paramount. As with all Ruskinn
workstations, the Concept Plus combines excellent performance and ergonomics
without compromising quality with a large 600 plate capacity.
Concept 1000 is the ultimate workstation. It has been designed for large
laboratories or research applications, The Concept 1000 boasts all the standard
features of Ruskinn technology with a couple of unique features included. The
Concept 1000 has a unique dual chamber system, which is accessed through a
central interlock. Each work chamber features independent control of temperature
and humidity and has independent user access so two people can work at the same
time. An optional gas mixing module can be interfaced with one chamber to create
two independent environments. The Concept 1000 has a 1000 plate capacity (500
plates in each cabinet) and an interlock transfer rate of 100 plates per 5
minutes. The Concept 1000 is a serious incubator for large workloads.
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Air trace Environmental sampler and the Air trace Compressed gas sampler combine
tried and tested technology with an innovative new design and state of the art
control. The Air trace family brings a modern look to slit to agar impingement
whilst offering a robust low maintenance solution to your sampling needs. While
the distance between the slit and the agar surface is set automatically, the
user has control over key variables such as plate rotation speed and area
exposed. The air flow rate is automatically set at a fixed rate of 28.3 litres
per minute. For
localised Environmental Monitoring, the Air trace Environmental is available
with an optional remote probe assembly. This allows validating sampling at point
of fill applications during manufacturing, in laminar flow cabinets or in
trace samplers operate by drawing in air or gas at a fixed flow rate.
The head of each sampler holds a 140/150 mm agar plate at a fixed
distance from a precision cut slit. The plate is rotated through 360 degrees for
a sampling period of 1 hour (although users can adjust sampling time and the
area of plate exposed). After appropriate incubation, the growth on the plated
medium can be interpreted not only quantitatively but also from a real time
occurrence of contamination. This can prove invaluable in pinpointing which
event during a manufacturing process or surgical operation have raised the
environmental bio-burden above acceptable levels. Once identified these areas
can be scrutinised and appropriately addressed.
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DILUTION & ENRICHMENT PRODUCTS:
in 1990, International BioProducts (IBP) environmental sampling, dilution and
enrichment products are now part of the Biotrace International’s growing
portfolio of industrial microbiology products. A perfect complement to the
group’s extensive offering of rapid microbiology products, the IBP traditional
microbiology range is innovatively designed for convenience based on our
customer’s recommendations and are manufactured with the best materials
available, to give the best value. The
IBP range helps meet internal quality standards and government regulations
through various applications:
specifically for microbial surface sampling, our innovative sponges offer
exceptional convenience and unsurpassed quality. Our swab products are carefully
selected for environmental sampling applications, incorporating
both solid design and exceptional ease of use. Products available: Hydrasponge,
Envirosponge, Spongesicle and Rediswabs.
A complete line of convenient, high quality, cost effective HACCP sampling
products. Products available: Megareg Cattle/Swine kits, Poultry Rinse and
Dilutions and Enrichments
time saving solution for the laboratory. Designed with a host of innovative
features, all products are stringently controlled for quality to ensure the very
best products for their customers. Products available: Fliptop Dilution Bottles,
Quick Dilute, Redibroth, Quick Enrich and BioPro Dehydrated Media.
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For more information on
Biotrace please visit their website at www.biotrace.com