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Grain field representing mycotoxin testing across the grain supply chain

Food Safety Insights | April 2026

Mycotoxin Testing and Prevention Across the Grain Supply Chain

Learn how mycotoxins impact grain quality and food safety, and explore best practices for testing, prevention, and supply chain control during harvest season.

Mycotoxin testing during harvest season is essential for maintaining grain quality and helping to ensure food and feed safety. Understanding mycotoxins is the first step in managing their risk across food and feed supply chains.

What Are Mycotoxins?

As the name suggests, mycotoxins are toxins produced by fungi, such as yeasts and moulds. Due to their varied nature, fungi, or their spores, can be found virtually everywhere. When growth conditions are right, they can multiply quickly and produce toxins as byproducts.

Specific growth conditions, including temperature, humidity, and organic food sources, have an impact on whether particular fungi will grow and on the characteristics of the toxin byproducts produced.

  • Aflatoxin
  • Fumonisin
  • Ochratoxin
  • T-2 / HT-2
  • Deoxynivalenol (DON)
  • Zearalenone

How Does Mycotoxin Contamination Occur?

Grains provide an ideal environment for fungal growth, making them vulnerable to mycotoxin contamination both pre-harvest in the field and post-harvest during storage. Factors such as insect damage, poor handling practices, and environmental stress can compromise the grain kernel, allowing fungi to invade and multiply.

Once established, these fungi may produce mycotoxins that persist throughout storage and processing, making early detection and monitoring critical.

Favourable conditions that can increase mycotoxin contamination risk
Environmental and handling factors can increase the risk of fungal growth and mycotoxin formation.

Preparing for Mycotoxin Testing During Harvest Season

As harvest time approaches, testing processes are often reviewed to help ensure the crop is safe and harvest runs as efficiently as possible. Understanding the nuances of mycotoxin testing is crucial to help protect food and feed quality and support regulatory compliance.

Particle sizeAchieve a suitable particle size for efficient extraction.
Reference materialUse mycotoxin reference material (MRM) to validate method performance.
Method complianceEnsure test methods comply with advisory and regulatory legislation.

Together, these elements support reliable results during a time when testing volumes often increase significantly.

Sampling Best Practice

Effective testing starts with representative sampling. Because mycotoxins are often unevenly distributed, a robust sampling plan is critical during harvest.

  • Obtain representative samples from each grain load.
  • Grind a sufficient quantity of the sample to a consistent particle size.
  • Test the recommended portion size of the ground sample.

These steps help ensure sample homogeneity, improving the accuracy and reliability of test results.

Proficiency Testing

Enrolment in a proficiency testing programme is another key element of a strong mycotoxin control strategy. The use of reference materials helps laboratories identify training needs, validate ongoing performance, and document method reliability.

By understanding the strengths and limitations of selected testing methods, laboratories can improve data quality and maintain confidence in their results throughout the testing season.

Committed to being more than a product provider, Neogen® offers mycotoxin reference material and a robust proficiency testing programme. If you would like to explore more about this offering, contact the team here.

Managing Mycotoxin Control Throughout the Supply Chain

Mycotoxin management does not stop at harvest. Responsibility for testing often shifts as grain moves from farmers and producers to buyers, transporters, mills, and processors.

Some businesses focus testing intensity around the new crop period, with reduced volumes throughout the year, while others operate year-round testing as part of continuous production. In both cases, aligning testing strategies with operational priorities is essential.

Consistent operational prioritiesConsistent mycotoxin testing plays a vital role in maintaining grain quality before, during, and after harvest, covering both raw materials and finished products.
Seasonal operational prioritiesSeasonal testing commonly peaks after harvest and supports rapid decisions on whether grain should be accepted, stored, valued, or redirected.

Preventing Mycotoxin Outbreaks

Testing plays an essential role in safeguarding crops, but preventing mycotoxin contamination remains the most effective approach to mycotoxin control.

Proper Drying and Storage

Drying grains promptly to safe moisture levels immediately after harvest significantly reduces the risk of fungal growth. Once dried, grains must be stored in conditions that prevent moisture reabsorption and minimise temperature fluctuations.

Cleaning and Sanitation

Effective cleaning practices reduce the presence and spread of mould spores throughout harvesting equipment, storage facilities, and processing environments. Maintaining high hygiene standards at every stage of the supply chain supports long-term mycotoxin risk reduction.

Regular Testing and Monitoring

Routine testing and monitoring enable early detection and effective management of mycotoxin contamination. Sampling should take place at multiple stages, including pre-harvest, post-harvest, during storage, and throughout processing.

Mycotoxin detection and analysis workflow
Rapid screening tools such as lateral flow tests and ELISA methods support fast, confident decision-making.

Rapid screening tools such as lateral flow tests and ELISA methods allow for fast, on-site decision-making. To find out more about the testing solutions and support Neogen can offer, please click here.

Key Takeaways

Effective mycotoxin control relies on understanding how contamination occurs, implementing robust testing during harvest, and maintaining preventative measures throughout storage and processing. By aligning sampling, testing, and prevention strategies across the supply chain, producers can protect grain quality, support compliance, and improve food and feed safety.

Explore ADP Mycotoxin Screening Solutions

View rapid mycotoxin screening kits and testing support available from ADP.

View Mycotoxin Products